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发表于 2019-6-4 18:36:22
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- s='''
- PMID- 31131053{PMID号}
- OWN - NLM
- STAT- In-Data-Review
- LR - 20190527
- IS - 1838-7640 (Electronic)
- IS - 1838-7640 (Linking)
- VI - 9
- IP - 9
- DP - 2019{年}
- TI - Regulation of ezrin tension by S-nitrosylation mediates non-small cell lung
- cancer invasion and metastasis.
- PG - 2555-2571
- LID - 10.7150/thno.32479 [doi]【可能会有两个LID,选择后面有[doi]的】{DOI号}
- AB - Cancer invasion and metastasis depend on accurate and rapid modulation of both
- chemical and mechanical activities. The S-nitrosylation (SNO) of membrane
- cytoskeletal cross-linker protein ezrin may regulate the malignant process in a
- tension-dependent manner. Methods: The level of nitrosylated ezrin in non-small
- cell lung cancer (NSCLC) tissues and A549 cell line were evaluated by
- biotin-switch assay. A few cysteine mutated plasmids of ezrin were used to
- identify active site for SNO. Newly designed ezrin or mutated-ezrin tension
- probes based on Forster resonance energy transfer (FRET) theory were applied to
- visually observe real-time tension changes. Cytoskeleton depolymerizing and motor
- molecular inhibiting experiments were performed to reveal the alternation of the
- mechanical property of ezrin after SNO. Transwell assays and xenograft mouse
- model were used to assess aggressiveness of A549 cells in different groups.
- Fluorescent staining was also applied to examine cellular location and
- structures. Results: High inducible nitric oxide synthase (iNOS) levels were
- observed to induce ezrin-SNO, and then promote malignant behaviors of NSCLC cells
- both in vitro and in vivo. Cys(117) was identified as the only active site for
- ezrin-SNO. Meanwhile, an increased level of ezrin tension was observed after
- iNOS-induced SNO. Enhanced ezrin tension was positively correlated with
- aggressiveness of NSCLC. Moreover, Microfilament (MF) forces instead of
- microtubule (MT) forces played dominant roles in modulating ezrin tension,
- especially after ezrin nitrosylation. Conclusion: This study revealed a
- SNO-associated mechanism underlying the mechanical tension of ezrin. Ezrin-SNO
- promotes NSCLC cells invasion and metastasis through facilitating mechanical
- transduction from the cytoskeleton to the membrane. These studies implicate the
- therapeutic potential by targeting ezrin in the inhibition NSCLC invasion and
- metastasis. {摘要}
- FAU - Zhang, Xiaolong
- AU - Zhang X
- AD - State Key Laboratory Cultivation Base for TCM Quality and Efficacy, School of
- Medicine and Life Science, Nanjing University of Chinese Medicine, Nanjing
- 210023, Jiangsu, PR China.
- AD - Key Laboratory of Drug Target and Drug for Degenerative Disease, Nanjing
- University of Chinese Medicine, Nanjing 210023, Jiangsu, PR China.
- FAU - Li, Guangming
- AU - Li G
- AD - Department of Anesthesiology, Huaian First People's Hospital, Nanjing Medical
- University, Huaian 223001, Jiangsu, PR China.
- FAU - Guo, Yichen
- AU - Guo Y
- AD - Department of Surgery and Biomedical Engineering, University of Alabama at
- Birmingham (UAB), Birmingham, Alabama. 35294, USA.
- FAU - Song, Ying
- AU - Song Y
- AD - Department of Respiratory Medicine, The First Affiliated Hospital of Nanjing
- Medical University, Nanjing 210029, PR China.
- FAU - Chen, Linlin
- AU - Chen L
- AD - State Key Laboratory Cultivation Base for TCM Quality and Efficacy, School of
- Medicine and Life Science, Nanjing University of Chinese Medicine, Nanjing
- 210023, Jiangsu, PR China.
- AD - Key Laboratory of Drug Target and Drug for Degenerative Disease, Nanjing
- University of Chinese Medicine, Nanjing 210023, Jiangsu, PR China.
- FAU - Ruan, Qinli
- AU - Ruan Q
- AD - State Key Laboratory Cultivation Base for TCM Quality and Efficacy, School of
- Medicine and Life Science, Nanjing University of Chinese Medicine, Nanjing
- 210023, Jiangsu, PR China.
- AD - Key Laboratory of Drug Target and Drug for Degenerative Disease, Nanjing
- University of Chinese Medicine, Nanjing 210023, Jiangsu, PR China.
- FAU - Wang, Yifan
- AU - Wang Y
- AD - State Key Laboratory Cultivation Base for TCM Quality and Efficacy, School of
- Medicine and Life Science, Nanjing University of Chinese Medicine, Nanjing
- 210023, Jiangsu, PR China.
- AD - Key Laboratory of Drug Target and Drug for Degenerative Disease, Nanjing
- University of Chinese Medicine, Nanjing 210023, Jiangsu, PR China.
- FAU - Sun, Lixia
- AU - Sun L
- AD - Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210023,
- Jiangsu, PR China.
- FAU - Hu, Yunfeng
- AU - Hu Y
- AD - State Key Laboratory Cultivation Base for TCM Quality and Efficacy, School of
- Medicine and Life Science, Nanjing University of Chinese Medicine, Nanjing
- 210023, Jiangsu, PR China.
- AD - Key Laboratory of Drug Target and Drug for Degenerative Disease, Nanjing
- University of Chinese Medicine, Nanjing 210023, Jiangsu, PR China.
- FAU - Zhou, Jingwen
- AU - Zhou J
- AD - The First Clinical Medical College of Nanjing University of Chinese Medicine,
- Nanjing, Jiangsu 210023, PR China.
- FAU - Ren, Bin
- AU - Ren B
- AD - Department of Surgery and Biomedical Engineering, University of Alabama at
- Birmingham (UAB), Birmingham, Alabama. 35294, USA.
- FAU - Guo, Jun【如果最后一个作者有2个单位,2个单位都要,但是可以放在同一个单元格中】{通讯作者全名(优先选择地址里面有邮箱的,否则就选择最后一个作者)}
- AU - Guo J
- AD - State Key Laboratory Cultivation Base for TCM Quality and Efficacy, School of
- Medicine and Life Science, Nanjing University of Chinese Medicine, Nanjing
- 210023, Jiangsu, PR China. {通讯作者单位}
- AD - Key Laboratory of Drug Target and Drug for Degenerative Disease, Nanjing
- University of Chinese Medicine, Nanjing 210023, Jiangsu, PR China.
- LA - eng
- PT - Journal Article
- DEP - 20190413{发表时间}
- PL - Australia
- TA - Theranostics
- JT - Theranostics{期刊全名}
- JID - 101552395
- PMC - PMC6525990
- OTO - NOTNLM
- OT - FRET
- OT - ezrin S-nitrosylation
- OT - invasion
- OT - metastasis
- OT - non-small cell lung cancer【所有OT,可以放在同一个单元格中】{关键词}
- COIS- Competing Interests: The authors have declared that no competing interest exists.
- EDAT- 2019/05/28 06:00
- MHDA- 2019/05/28 06:00
- CRDT- 2019/05/28 06:00
- PHST- 2018/12/21 00:00 [received]
- PHST- 2019/03/18 00:00 [accepted]
- PHST- 2019/05/28 06:00 [entrez]
- PHST- 2019/05/28 06:00 [pubmed]
- PHST- 2019/05/28 06:00 [medline]
- AID - 10.7150/thno.32479 [doi] 【可能会有两个AID,选择后面有[doi]的】{DOI号2}
- AID - thnov09p2555 [pii]
- PST - epublish
- SO - Theranostics. 2019 Apr 13;9(9):2555-2571. doi: 10.7150/thno.32479. eCollection
- 2019. {引文信息}
- '''
-
- import re
- pmid=re.search("PMID- (\d+){",s)
- print(pmid.group(1))
- dp=re.search("DP\s+- (\d+){",s)
- print(dp.group(1))
- ti= re.search("TI\s+- ([\sa-zA-Z-\.]+)PG",s,re.M)
- print(ti.group(1))
- lid=re.search("LID\s+- ([\sa-zA-Z-\.\\0-9]+)\[",s)
- print(lid.group(1))
- ab=re.search("AB\s+- ([\sa-zA-Z-\.\\0-9\(\),:]+){",s,re.M)
- print(ab.group(1))
- fau=re.findall("FAU\s+- ([\sa-zA-Z,]+)$",s,re.M)
- for x in fau:print(x)
- ad= re.search("AD\s+- ([\sa-zA-Z0-9-\.,]+){",s,re.M)
- print(ad.group(1))
- dep=re.search("DEP - (\d+){",s)
- print(dep.group(1))
- jt=re.search("JT\s+- ([A-Za-z]+){",s)
- print(jt.group(1))
- ot=re.findall("OT\s+- ([A-Za-z- ]+)$",s,re.M)
- for x in ot:print(x)
- ot2=re.search("OT\s+- ([a-z]+\-[a-z]+\s+[a-z]+\s+[a-z]+\s+[a-z]+)",s)
- print(ot2.group(1))
- aid=re.findall("AID\s+- ([\sa-z0-9\.\/]+)",s)
- for x in aid:print(x)
- so=re.search("SO\s+- ([\sA-Za-z0-9\.\/:;\(\)-]+)",s,re.M)
- print(so.group(1))
-
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